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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/10/2014 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ALEM, D.; DIAZ, P.; LEONI, C.; DE SIMONE, S.G.; CORREA, A.; OPPEZZO, P.; DALLA RIZZA, M. |
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Afiliación : |
DIEGO MARTIN ALEM GLISON, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; PAOLA ALEXANDRA DIAZ DELLAVALLE, Departamento de Ciencia y Tecnología de los Alimentos, Facultad de Ingeniería y Tecnologías, Universidad Católica del Uruguay (UCU), Montevideo, Uruguay; CAROLINA LEONI VELAZCO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
In Search of Topical Agricultural Biofungicides: Properties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Journal of Microbial & Biochemical Technology, 2014, v. 6, no.5, p. 268-273. |
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ISSN : |
1948-5948 |
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DOI : |
10.4172/1948-5948.1000155 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability. MenosABSTRACT.
Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an
interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP
was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For
topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resista... Presentar Todo |
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Thesagro : |
ACTIVIDAD ANTIMICROBIANA; ALTERNARIA; BIOTECNOLOGÍA; PÉPTIDOS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/3627/1/Dalla-Rizza-M.-2014.-Jr.Microb.Biochem.-Technol.-v.65.pdf
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Marc : |
LEADER 02624naa a2200265 a 4500
001 1051279
005 2019-10-15
008 2014 bl uuuu u00u1 u #d
022 $a1948-5948
024 7 $a10.4172/1948-5948.1000155$2DOI
100 1 $aALEM, D.
245 $aIn Search of Topical Agricultural Biofungicides$bProperties of the Recombinant Antimicrobial Peptide TrxAq-AMP obtained from Amaranthus quitensis.$h[electronic resource]
260 $c2014
520 $aABSTRACT. Synthetic pesticides have a positive impact on food production. However, there are concerns due to the outbreak of resistance along with negative side effects on human health and the environment. New active compounds and control strategies are needed for the management of phytopathogens. Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune response in almost all organisms that constitute an interesting source of potential molecules for use as pesticides. The naturally derived antimicrobial peptide Aq- AMP (Amaranthus quitensis-Antimicrobial Peptide), obtained from Amaranthus quitensis, is cysteine-rich with activity against several phytopathogens. In the present work, we report on the expression in Escherichia coli of functionally active Aq-AMP fused to thioredoxin (TrxAq-AMP). The in vitro antifungal activity of purified TrxAq-AMP was confirmed against Alternaria solani, Fusarium oxysporum f. sp. lycopersici, Pencillium digitatum and P. italicum, as well as the in vivo control of P. digitatum in oranges. We demonstrated the stability of TrxAq-AMP in a range of pH (from 3 to 11) and at temperatures from 0°C to 100°C. Furthermore, it maintained activity after digestion with various proteases and it displayed no haemolytic activity suggesting a highly stable and safe molecule. For topical application we present AMP with no haemolytic/phytotoxic activity that is effective, stable to a wide range of temperature and pH, and resistant to protease activity. Besides, this molecule is naturally stored in the seed, easy to extract and potentially produced through molecular farming. These findings encourage further biotechnological research on topical application of AMPs, especially in relation to molecule bioavailability.
650 $aACTIVIDAD ANTIMICROBIANA
650 $aALTERNARIA
650 $aBIOTECNOLOGÍA
650 $aPÉPTIDOS
700 1 $aDIAZ, P.
700 1 $aLEONI, C.
700 1 $aDE SIMONE, S.G.
700 1 $aCORREA, A.
700 1 $aOPPEZZO, P.
700 1 $aDALLA RIZZA, M.
773 $tJournal of Microbial & Biochemical Technology, 2014$gv. 6, no.5, p. 268-273.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Salto Grande. |
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Fecha actual : |
28/06/2018 |
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Actualizado : |
28/06/2018 |
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Tipo de producción científica : |
Documentos |
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Autor : |
RUBIO, L.; ALVES, P.; AMARAL, J.; BLANCO, O.; GUIMARAENS, A. |
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Afiliación : |
LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO DANIEL ALVES MENONI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN ANTONIO AMARAL SORIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORIBE BLANCO MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDREA ELIZABETH GUIMARAENS SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Efecto de las aplicaciones de verano con productos alternativos a los cobres convencionales sobre el control de cancro cítrico y daños cosméticos de la fruta. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
In: INIA Salto Grande; Programa Nacional Producción Citrícola. Avances de investigación en protección vegetal citrícola. Salto (UY): INIA, 2013. |
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Páginas : |
p. 9-12 |
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Serie : |
(Serie Actividades de Difusión; 716) |
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ISSN : |
1688-9258 |
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Idioma : |
Español |
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Contenido : |
Las aplicaciones de cobre con altas temperaturas son problemáticas por el manchado que generan en la fruta de algunas mandarinas y naranjas Navel. No obstante, el cobre es actualmente la única opción para el control de cancro.El objetivo de esta actividad ha sido incorporar el uso de productos con bajo porcentaje de cobre (?inductores de resistencia?) en aplicaciones de enero y febrero y evaluar su efecto sobre el control de cancro cítrico y daños en la cosmética de la fruta. |
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Thesagro : |
CANCRO CITRICO. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10558/1/sad-716-p.-9-12.pdf
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Marc : |
LEADER 01285naa a2200217 a 4500
001 1058759
005 2018-06-28
008 2013 bl uuuu u00u1 u #d
022 $a1688-9258
100 1 $aRUBIO, L.
245 $aEfecto de las aplicaciones de verano con productos alternativos a los cobres convencionales sobre el control de cancro cítrico y daños cosméticos de la fruta.$h[electronic resource]
260 $c2013
300 $ap. 9-12
490 $a(Serie Actividades de Difusión; 716)
520 $aLas aplicaciones de cobre con altas temperaturas son problemáticas por el manchado que generan en la fruta de algunas mandarinas y naranjas Navel. No obstante, el cobre es actualmente la única opción para el control de cancro.El objetivo de esta actividad ha sido incorporar el uso de productos con bajo porcentaje de cobre (?inductores de resistencia?) en aplicaciones de enero y febrero y evaluar su efecto sobre el control de cancro cítrico y daños en la cosmética de la fruta.
650 $aCANCRO CITRICO
700 1 $aALVES, P.
700 1 $aAMARAL, J.
700 1 $aBLANCO, O.
700 1 $aGUIMARAENS, A.
773 $tIn: INIA Salto Grande; Programa Nacional Producción Citrícola. Avances de investigación en protección vegetal citrícola. Salto (UY): INIA, 2013.
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INIA Salto Grande (SG) |
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